Stone fragmentation and basket extraction is suggested to have the advantage of a better SFR . In the present study, the SFR of Group F (78.6%) was significantly better than Group D (58.6%). ... In a randomised multicentre study conducted by Humphrey et al., similar results were reported for SFR (74.3% for fragmentation and basketting vs 58.2% ...
This systematic review infers that urologists can safely use either option of fragmentation and basket extraction or dusting without extraction to achieve similar outcomes as both techniques are similar for efficacy and safety.
Among the fragmentation-based approaches, Snyder et al. (2016) ... Cell-free plasma DNA extraction, sequencing, and data preprocessing.
Conclusions: This systematic review infers that urologists can safely use either option of fragmentation and basket extraction or dusting without extraction to achieve similar outcomes as both ...
This detailed fragmentation information enables disambiguation between multiple compositional possibilities for glycopeptide structure. Among the protein extraction and glycopeptide enrichment methods compared, EtOH precipitation and HILIC-SPE most efficiently extracted proteins in human milk and enriched tryptic N-glycopeptides, …
Shekhar et al. [Citation 16] suggest ... The relationship between Class 1 fragmentation and extraction ratio is not significant after extraction; the p-value is much higher than the significance level of 0.05, so the null hypothesis is valid. The data show a lot of variation as well (R 2 = 0.025). Similar behaviour can be observed for Class 2.
Fragmentation effects propagate through the whole ecosystem. (A to C) For each fragmentation treatment [reduced area in BDFFP, Wog Wog, Kansas (A); increased isolation in SRS and Moss (B); and increased edge in all experiments (C)], we summarize major findings for ecological processes at all levels of ecological organization. Each dot ...
This process of DNA extraction—collecting samples, lysing tissue and purifying or isolating target DNA—is the most time and labour-intensive step of most DNA tests and typically …
Liu X, Li P, Widlak P et al. DFF40 induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998; 95 :8461–6. Article CAS PubMed PubMed Central Google Scholar
A new assay for cell-free DNA fragmentation improves prediction of gene expression in liquid biopsies. ... Esfahani et al. 1 show that the expression level of genes captured in cfDNA samples can ...
Based on previous data demonstrating the utility of cell‐free DNA (cfDNA) fragmentation patterns (Mouliere et al, 2018a; Mouliere et al, 2018b; van der Pol & Mouliere, 2019), we …
An overview of the workflow and variables tested in this study are shown in Fig. 1.We evaluated MS/MS results acquired from EThcD-fragmentation technique through comparison of HCD- and ETD-derived MS/MS spectra, the efficiency of human milk protein extraction using EtOH precipitation, TCA precipitation and filtration using the 10-kDa …
Forest fragmentation is a major driver of global biodiversity loss and ecosystem degradation 1,2,3,4.Identifying which areas have the most severe forest fragmentation is a fundamental task in ...
Abstract Introduction: Ureteroscopic laser lithotripsy is becoming the most commonly utilized treatment for patients with urinary calculi. The Holmium:YAG (yttrium aluminum garnet) laser is integral to the operation and is the preferred flexible intracorporeal lithotrite. In recent years, there has been increasing interest in examining the effect of varying the …
INTRODUCTION. Destruction and degradation of natural ecosystems are the primary causes of declines in global biodiversity ( 1, 2 ). Habitat destruction typically leads to …
Comparison and outcomes of dusting versus stone fragmentation and extraction in retrograde intrarenal surgery: results of a systematic review and meta-analysis ... Pietropaolo et al. have shown how high-power Holmium lasers have brought to the endourology armamentarium the ability to select high-frequency laser pulses while …
Mitochondria contain their own genomes that, unlike nuclear genomes, are inherited only in the maternal line. Owing to a high mutation rate and low levels of recombination of …
Mitochondria contain their own genomes that, unlike nuclear genomes, are inherited only in the maternal line. Owing to a high mutation rate and low levels of recombination of mitrochondrial DNA ...
sécurité du travail et, en général, l'efficacité des travaux à ciel ouvert. Dans la plupart des cas. la préparation des roches à l'extraction repose sur la destruction du massif des roches jusqu'à l'obtention de morceaux de dimensions néces saires et admissibles pour le travail normal de tout le complexe d'extraction et de transport.
Johnston, A.D., Lu, J., Korbie, D. et al. Modelling clinical DNA fragmentation in the development of universal PCR-based assays for bisulfite-converted, formalin-fixed and …
Background Specific applications and modern technologies, like non-invasive prenatal testing, non-invasive cancer diagnostic and next generation sequencing, are currently in …
1. Introduction. Besides solid phase, bead based and phenol based isolation methods, electrophoretic nucleic acid extraction has tended to be sidelined and has been used mainly for short RNA targets (Vulto et al., 2010).However in life science genomic DNA is an essential target, because it is relatively stable and can be extracted from organic …
INTRODUCTION. Sperm DNA fragmentation (SDF) refers to single-stranded or double-stranded breaks in the genome of spermatozoa. Because the mature male gamete lacks the ability to repair DNA damage [], these breaks tend to persist and can negatively influence male reproductive potential and outcomes.Three primary mechanisms can lead to SDF: …
Satellite data and modelling reveal that tropical forest fragments have similar size distributions across continents, and that forest fragmentation is close to a critical point, beyond which ...
Fragmentation et extraction des reins polykystiques au cours de la néphrectomie cœlioscopique manuellement assistée : une étude observationnelle prospective @article{Magnier2021FragmentationEE, title={Fragmentation et extraction des reins polykystiques au cours de la n{'e}phrectomie c{oe}lioscopique manuellement …
Esteves et al. found testicular sperm to have a three to five times lower proportion of DNA fragmentation than ejaculated sperm. In a recent prospective comparative study on the use of testicular sperm for ICSI in 172 patients with high levels of sperm DNA damage, it was found that the proportion of testicular sperm DNA damage (8.3%) was five ...
Ecologists agree that habitat destruction is detrimental to the maintenance of biodiversity, but they disagree—often strongly—on the extent to which fragmentation itself is to blame (4, 5).Early hypotheses based on the biogeography of oceanic islands provided a theoretical framework to understand fragmentation's effect on extinction in terrestrial landscapes …
Unlike most short-read sequencing techniques, which only require very small amounts of DNA, the extraction of inhibitor-free, high molecular weight DNA, which is suitable for long-read sequencing, has always been a challenge due to …
Circulating cell-free DNA (cfDNA) is a pool of short DNA fragments mainly released from apoptotic hematopoietic cells. Nevertheless, the precise physiological process governing the DNA fragmentation and molecular profile of cfDNA remains obscure. To dissect the DNA fragmentation process, we use a human leukemia cell line HL60 undergoing …
cause of fragmentation (Allentoft . et al., 2012). By adjusting for this baseline of degradation other, more situational, factors for degradation such as the influence of …
The Shokere et al. group suggested that the concentration of DNA increased slightly with increasing fragmentation of DNA. However, our results show that DNA quantification based on A 260 is not significantly affected by the …
Microbial DNA extraction and sequencing. We extracted total microbial DNA from 0.25 g of soil per site using an E.Z.N.A DNA Extraction Kit (D5625-02; Omega Bio-tek, Norcross, GA, USA). ... For instance, how plant–microbe interactions are affected by edge effects, a well-established consequence of habitat fragmentation (Ries et al., 2004), has ...
Tous les malades avaient bénéficié d'une méatotomie endoscopique horizontale avec fragmentation et extraction des calculs. Le temps opératoire moyen a été de 27 mn (18-58).
Oocytes and early embryos have been shown to repair sperm DNA damage, so the effect of sperm DNA fragmentation depends on the combined effects of sperm chromatin …
Isoflavonoid derivatives such as pterocarpans show much more complex fragmentation pathways; MS studies of different deuterated peterocarpan derivatives, as well as of pterocarpan glycosides, points out that these are dominated by various and successive ring openings and/or contractions (Zhang et al. 2007).A rare 2/4 rDA fragmentation, like …
In fragmented DNA, PCR-based methods quantify the number of intact regions at a specific amplicon length. However, the relationship between the population of DNA fragments within a sample and the ...